Journal Article

Antimicrobial Role of RNASET2 Protein During Innate Immune Response in the Medicinal Leech Hirudo verbana

Orlandi, Viviana Teresa, de Eguileor, Magda, Monti, Laura, Baranzini, Nicolò, Reguzzoni, Marcella, Rosini, Elena, Tettamanti, Gianluca, Acquati, Francesco, Grimaldi, Annalisa, De Vito, Annarosaria, Pollegioni, Loredano

2020

Overview

The innate immune response represents a first-line defense against pathogen infection that has been widely conserved throughout evolution. Using the invertebrate (Annelida, Hirudinea) as an experimental model, we show here that the RNASET2 ribonuclease is directly involved in the immune response against Gram-positive bacteria. Injection of lipoteichoic acid (LTA), a key component of Gram-positive bacteria cell wall, into the leech body wall induced a massive migration of granulocytes and macrophages expressing TLR2 (the key receptor involved in the response to Gram-positive bacteria) toward the challenged/inoculated area. We hypothesized that the endogenous leech RNASET2 protein ( RNASET2) might be involved in the antimicrobial response, as already described for other vertebrate ribonucleases, such as RNase3 and RNase7. In support of our hypothesis, RNASET2 was mainly localized in the granules of granulocytes, and its release in the extracellular matrix triggered the recruitment of macrophages toward the area stimulated with LTA. The activity of RNASET2 was also evaluated on living cells by means of light, transmission, and scanning electron microscopy analysis. RNASET2 injection triggered the formation of clumps following a direct interaction with the bacterial cell wall, as demonstrated by immunogold assay. Taken together, our data support the notion that, during the early phase of leech immune response, granulocyte-released RNASET2 triggers bacterial clumps formation and, at the same time, actively recruits phagocytic macrophages in order to elicit a rapid and effective eradication of the infecting microorganisms from inoculated area.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Animals / antimicrobial activity / Bacteria / Bacterial infections / Body wall / CD11b Antigen - physiology / Cell walls / Defense / Enzymes / Extracellular matrix / Gram-negative bacteria / Gram-positive bacteria / Hirudo medicinalis - immunology / Hirudo verbana / Immune response / Immunity, Innate / Immunology / Innate immunity / Invertebrates / Leukocyte migration / Leukocytes (granulocytic) / Lipopolysaccharides - pharmacology / Lipoteichoic acid / LTA / Macrophages / Macrophages - immunology / medicinal leech / Membranes / Pathogens / Phagocytes / Phagocytosis / Proteins / Ribonucleases - physiology / RNASET2 / Scanning electron microscopy / Staphylococcus aureus / Teichoic Acids - pharmacology / TLR2 protein / Toll-Like Receptor 2 - physiology / Toll-like receptors / Vertebrates / Yeast