Asset Details
Do you wish to reserve the book?
Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing
Do you wish to request the book?
Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Snapshot study of canine distemper virus in Bangladesh with on-site PCR detection and nanopore sequencing
2024
Overview
Canine distemper virus (CDV) is a highly contagious virus that affects domestic and wild animals, causing severe illness with high mortality rates. Rapid monitoring and sequencing can provide valuable information about circulating CDV strains, which may foster effective vaccination strategies and the successful integration of these into conservation programs. During two site visits in Bangladesh in 2023, we tested a mobile, deployable genomic surveillance setup to explore the genetic diversity and phylogenetic patterns of locally circulating CDV strains. We collected and analysed 355 oral swab samples from stray dogs in Rajshahi and Chattogram cities, Bangladesh. CDV-specific real-time RT-PCR was performed to screen the samples. Out of the 355 samples, 7.4% (10/135) from Rajshahi city and 0.9% (2/220) from Chattogram city tested positive for CDV. We applied a real-time RT-PCR assay and a pan-genotype CDV-specific amplicon-based Nanopore sequencing technology to obtain the near-completes. Five near-complete genome sequences were generated, with phylogenetic relation to the India-1/Asia-5 lineage previously identified in India. This is the first study to provide genomic data on CDV in Bangladesh and the first demonstration of a mobile laboratory setup as a powerful tool in rapid genomic surveillance and risk assessment for CDV in low resource regions.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/699
/ Animals
/ Distemper Virus, Canine - classification
/ Distemper Virus, Canine - genetics
/ Distemper Virus, Canine - isolation & purification
/ Dogs
/ Genomics
/ Genotype
/ Humanities and Social Sciences
/ Nanopore Sequencing - methods
/ Real-Time Polymerase Chain Reaction - methods
/ Science
/ Viruses
