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A high-performance thin-layer chromatography densitometric method for the separation of isomeric ceftriaxone in powder for injection formulation
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A high-performance thin-layer chromatography densitometric method for the separation of isomeric ceftriaxone in powder for injection formulation
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Journal Article
A high-performance thin-layer chromatography densitometric method for the separation of isomeric ceftriaxone in powder for injection formulation
2024
Overview
The aim of this study was to develop and validate a High-Performance Thin Layer Chromatographic (HPTLC) method for simultaneous determination of ceftriaxone and ceftriaxone e-isomer in powder for injection formulation. Ceftriaxone sodium injection is an antibiotic that used globally. It has Z/E geometrical conformation, in which ceftriaxone sodium and 3 ene-isomer have Z- conformation while (E)-isomer has E- conformation and the potential toxicity of ceftriaxone (E)-isomer has been reported. Thus, to safeguard the public health, a simple and easy to use, rapid and reliable method was developed for qualitative and quantitative determination of ceftriaxone sodium and its (E)–isomer. Samples were applied on HPTLC glass plates precoated with silica gel 60F254 by using Linomat semi-auto sampler. Separation was carried out using acetone, triethyl amine, water, chloroform and ethyl acetate as a mobile phase in different ratios. The R
f
values of separated compounds were 0.51 ± 0.01 and 0.62 ± 0.01 for ceftriaxone sodium and ceftriaxone (E)-isomer respectively. The method was validated by studying Specificity, Linearity, Accuracy, Precision, Robustness, Limit of Detection (LOD) and Limit of Quantification (LOQ) and Solution stability. The developed method was successfully, sensitive, simple, precise, accurate, robust and applicable for the simultaneous determination of ceftriaxone sodium and ceftriaxone (E)-isomer in powder for injection formulation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 692/308
/ Analytical method validation
/ Anti-Bacterial Agents - analysis
/ Anti-Bacterial Agents - chemistry
/ Chromatography, High Pressure Liquid - methods
/ Chromatography, Thin Layer - methods
/ High performance thin layer chromatography
/ Humanities and Social Sciences
/ Isomers
/ Powder
/ Powders
/ Science
/ Silica
/ Sodium
/ Toxicity
