Journal Article

Detection of Cytotoxic Activity of Lectin on Human Colon Adenocarcinoma (Sw480) and Epithelial Cervical Carcinoma (C33-A)

Esquivel-Soto, Jaime, García-Carrancá, Alejandro, Zuñiga-Pérez, Clara, Alvarez-Manilla, Gerardo, Villagómez-Ibarra, Roberto, Madrigal-Santillán, Eduardo, Esquivel-Chirino, Cesar, Riverón-Negrete, Leticia, Valadez-Vega, Carmen, Morales-González, José A., Morales-González, Ángel, Bautista, Mirandeli

2011

Overview

Lectins comprise a heterogeneous class of proteins that recognize the carbohydrate moieties of glycoconjugates with high specificity. Numerous studies have shown that lectins are capable of recognizing specific carbohydrate moieties displayed by malignant cells or tissues. The present work was performed to investigate the effects of tepary bean (Phaseolus acutifolius) lectins on proliferation, colony formation, and alteration of DNA synthesis of human malignant cells. Tepary bean lectin showed dose dependent effects on the inhibition of viability as well as on colony formation in two human malignant cells lines (C33-A, Sw480); By contrast, tepary bean lectin only showed significant effects on DNA synthesis on Sw480 cells. Our results provide evidence of the anti- proliferative and cytotoxic effects of the tepary bean lectins on C33-A and Sw480 cells lines.

• Publisher: MDPI AG,MDPI
• Subject: Adenocarcinoma - pathology / Cancer / Carbohydrates / Cell growth / Cell Proliferation - drug effects / Colonic Neoplasms - pathology / Cytotoxicity / Disease prevention / Drug Screening Assays, Antitumor / Electronic mail systems / Electrophoresis, Polyacrylamide Gel / Female / Humans / Lectins / Lectins - pharmacology / Neoplasms, Glandular and Epithelial - pathology / tepary bean lectins / tepary beans / Uterine Cervical Neoplasms - pathology