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CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana
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CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana
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CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana
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Journal Article
CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana
2025
Overview
Key message
Tobacco etch virus
accumulation declined in
Nicotiana benthamiana eEF1Bγ
gene-edited lines, suggesting that eEF1Bγ may be a host factor for this virus.
Viruses use host factors to replicate and move from cell to cell. Therefore, the editing of genes encoding viral host factors that are not essential for plant survival enables the rapid development of plants with durable virus resistance. Eukaryotic initiation factors, such as eIF4E and eIF4G, function as host factors for viral infection, and loss-of-function mutations of these factors lead to virus resistance. Broadening the spectrum of host factor targets would help expand resources for engineering virus resistance. In this study, we tested whether editing the eukaryotic translation elongation factor gene
eEF1Bγ
would produce virus-resistant plants. Accordingly, we targeted the four
eEF1Bγ
genes in
Nicotiana benthamiana
for editing using virus-induced gene editing (VIGE) with
Tobacco rattle virus
(TRV). Although we attempted to obtain plants edited for all four
eEF1Bγ
homologs, we failed to identify such plants. Instead, we obtained plants with three of the four homologs knocked out, harboring 1-bp insertion/deletions resulting in premature stop codons. These
eEF1Bγ
-edited plants did not exhibit resistance to
Potato virus X
(PVX),
Tobacco mosaic virus
(TMV), or
Tomato bushy stunt virus
(TBSV) but showed reduced accumulation of
Tobacco etch virus
(TEV) compared to wild-type plants. These findings demonstrate the feasibility of conferring resistance in plants through gene editing of
eEF1Bγ
, underscoring the importance of exploring diverse host factor targets for comprehensive virus resistance.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
