Journal Article

Altered Monocyte and Lymphocyte Phenotypes Associated with Pathogenesis and Clinical Efficacy of Progestogen Therapy for Peritoneal Endometriosis in Adolescents

Kiseleva, Viktoriia V., Fatkhudinov, Timur Kh, Poltavets, Anastasiya S., Vtorushina, Valentina V., Gantsova, Elena A., Chuprynin, Vladimir D., Khashchenko, Elena P., Uvarova, Elena V., Krechetova, Lyubov V., Vishnyakova, Polina A., Elchaninov, Andrey V., Inviyaeva, Eugeniya V., Sukhikh, Gennady T.

2024

Overview

Background: Immunological imbalances characteristic of endometriosis may develop as early as the primary manifestations of the disease in adolescence. Objective: To evaluate subpopulation dynamics of monocytes and lymphocytes in peripheral blood and peritoneal fluid of adolescents with peritoneal endometriosis at diagnosis and after 1-year progestogen therapy. Methods: This study included 70 girls, 13–17 years old, diagnosed laparoscopically with peritoneal endometriosis (n = 50, main group) or paramesonephric cysts (n = 20, comparison group). Phenotypes of monocytes and lymphocytes of the blood and macrophages of the peritoneal fluid were analyzed by flow cytometry at diagnosis and during progestogen therapy. Results: Differential blood counts of CD16+ (p < 0.001) and CD86+ (p = 0.017) monocytes were identified as independent risk factors for peritoneal endometriosis in adolescents. During the treatment, cytotoxic lymphocytes CD56dimCD16bright (p = 0.049) and CD206+ monocytes (p < 0.001) significantly increased while CD163+ monocytes decreased in number (p = 0.017). The CD56dimCD16bright blood counts before (p < 0.001) and during progestogen therapy (p = 0.006), as well as CD206+ blood counts during the treatment (p = 0.038), were associated with the efficacy of pain relief after 1-year progestogen therapy. Conclusions: Adolescents with peritoneal endometriosis have altered counts of pro- and anti-inflammatory monocytes and lymphocytes both before and after 1-year progestogen therapy, correlating with treatment efficacy and justifying long-term hormonal therapy.

• Publisher: MDPI AG
• Subject: Adolescence / Adolescent / Adolescents / Ascitic Fluid / B cells / Care and treatment / CD16 antigen / CD163 / CD163 antigen / CD206 / CD86 antigen / Congenital diseases / Cysts / Cytotoxicity / Diagnosis / Disease / Endometriosis / Endometriosis - drug therapy / Endometriosis - pathology / Ethylenediaminetetraacetic acid / Female / Flow cytometry / Fluid flow / Genetic aspects / Gynecology / Humans / Informed consent / Laparoscopy / lymphocyte / Lymphocytes / Lymphocytes - drug effects / Lymphocytes - metabolism / macrophage / Macrophages / Monoclonal antibodies / Monocytes / Monocytes - drug effects / Monocytes - metabolism / Pathogenesis / Patients / pelvic pain / Peripheral blood / Peritoneal fluid / Peritoneum / Phenotype / Phenotypes / Progestins - pharmacology / Progestins - therapeutic use / Risk factors / Teenagers / Treatment Outcome / Youth