Asset Details
Do you wish to reserve the book?
Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
Do you wish to request the book?
Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
2008
Overview
Genetic transformation of wheat, using biolistics or
Agrobacterium
, underpins a range of specific research methods for identifying genes and studying their function
in planta
. Transgenic approaches to study and modify traits in durum wheat have lagged behind those for bread wheat. Here we report the use of
Agrobacterium
strain AGL1, with additional
vir
genes housed in a helper plasmid, to transform and regenerate the durum wheat variety Ofanto. The use of the basic pSoup helper plasmid with no additional
vir
genes failed to generate transformants, whereas the presence of either
vir
G
542
or the 15 kb Komari fragment containing
vir
B,
vir
C and
vir
G
542
produced transformation efficiencies of between 0.6 and 9.7%. Of the 42 transgenic plants made, all but one (which set very few seeds) appeared morphologically normal and produced between 100 and 300 viable seeds. The transgene copy number and the segregation ratios were found to be very similar to those previously reported for bread wheat. We believe that this is the first report describing successful genetic transformation of tetraploid durum wheat (
Triticum turgidum
L. var.
durum
) mediated by
Agrobacterium tumefaciens
using immature embryos as the explant.
Publisher
Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Agrobacterium tumefaciens - genetics
/ Animal Genetics and Genomics
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Fundamental and applied biological sciences. Psychology
/ Methods. Procedures. Technologies
/ Plants, Genetically Modified
/ plasmids
/ seeds
We currently cannot retrieve any items related to this title. Kindly check back at a later time.