Asset Details
Do you wish to reserve the book?
Electrostatic properties of disordered regions control transcription factor search and pioneer activity
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Electrostatic properties of disordered regions control transcription factor search and pioneer activity
Do you wish to request the book?
Electrostatic properties of disordered regions control transcription factor search and pioneer activity
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Electrostatic properties of disordered regions control transcription factor search and pioneer activity
2026
Overview
Transcription factors (TFs) search for and bind specific DNA target sites to control gene expression. However, eukaryotic TF target search within the chromatinized genome is poorly characterized. Here, we combine in vivo and in vitro single-molecule imaging to dissect the role of differentially-charged DNA-binding domain flanking regions in the target search of Sox TFs. We demonstrate that the flanking region of Sox2 markedly enhances search efficiency compared to the negatively-charged flanking region of Sox17. The involved mechanisms are distinct for naked DNA compared to chromatin. On DNA, the enhanced search of Sox2 is driven by an increased target recognition rate during 1D sliding, despite reduced sliding speed. Conversely, enhanced nonspecific interactions between the Sox2’s DNA-binding domain flanking region and nucleosomes facilitate binding to compact chromatin and reinforce pioneer activity. These findings provide critical insights into biophysical mechanisms governing TF target search within the chromatinized genome.
This work describes how positive charge in the peptide chain flanking the DNA-binding domain of transcription factors speeds up their search on free and chromatinized DNA, helping to explain why some transcription factors act as stronger pioneers.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/34
/ 45
/ 45/15
/ 45/23
/ 45/71
/ Animals
/ Binding
/ DNA
/ Genomes
/ Humanities and Social Sciences
/ Humans
/ Science
/ Sliding
/ SOXB1 Transcription Factors - chemistry
/ SOXB1 Transcription Factors - genetics
/ SOXB1 Transcription Factors - metabolism
/ SOXF Transcription Factors - chemistry
/ SOXF Transcription Factors - genetics
