Asset Details
Do you wish to reserve the book?
Discontinuous single electrode voltage‐clamp measurements: assessment of clamp accuracy in Vicia faba guard cells
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Discontinuous single electrode voltage‐clamp measurements: assessment of clamp accuracy in Vicia faba guard cells
Do you wish to request the book?
Discontinuous single electrode voltage‐clamp measurements: assessment of clamp accuracy in Vicia faba guard cells
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Discontinuous single electrode voltage‐clamp measurements: assessment of clamp accuracy in Vicia faba guard cells
2001
Overview
The use of a discontinuous single electrode voltage‐clamp (dSEVC) offers an attractive alternative to the patch‐clamp technique, since whole‐cell measurements can be performed with a single sharp electrode. Comparison of current–voltage relations, however, revealed a weaker voltage dependence of channels measured with the dSEVC compared to patch clamp. The accuracy of the dSEVC was tested on Vicia faba guard cells impaled with double‐barrelled electrodes. The actual clamp potential was measured independently of the dSEVC, at the second barrel. The weaker voltage dependence of ion channels appeared to be due to an overestimation of the clamp potential by the dSEVC. The deviation between the intended and actual clamp potential showed a linear relationship with the injected current; on average a 126 mV deviation was found for a clamp current of 1 nA. The deviation was probably caused by a slow settling capacity at the electrode, not compensated by the dSEVC amplifier. It is concluded that the dSEVC method in its current state is only suited for the study of small ion conductances in plant cells.
Publisher
Oxford University Press,OXFORD UNIVERSITY PRESS
Subject
/ Biological and medical sciences
/ Current voltage characteristics
/ Discontinuous single electrode voltage clamp
/ Fabaceae
/ Fundamental and applied biological sciences. Psychology
/ Kinetics
/ methods
/ Patch-Clamp Techniques - methods
/ Plant Epidermis - physiology
/ Plant physiology and development
/ Plant Structures - physiology
