Asset Details
Do you wish to reserve the book?
Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
Do you wish to request the book?
Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens
2020
Overview
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China, in December 2019. The virus rapidly spread globally, resulting in a public health crisis including almost 5 million cases and 323,256 deaths as of May 21, 2020. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected FFPE cell pellets. We identified a suitable rabbit polyclonal anti-SARS-CoV spike protein antibody and a mouse monoclonal anti-SARS-CoV nucleocapsid protein (NP) antibody for cross-detection of the respective SARS-CoV-2 proteins by IHC and immunofluorescence assay (IFA). Next, we established RNAscope in situ hybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex FISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. It is hoped that these reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models.
Publisher
American Society for Clinical Investigation
Subject
/ Animals
/ Antibodies, Viral - immunology
/ Antigens
/ Antigens, Viral - isolation & purification
/ Betacoronavirus - immunology
/ Betacoronavirus - isolation & purification
/ Clinical Laboratory Techniques - methods
/ Coronavirus Infections - diagnosis
/ Coronavirus Infections - pathology
/ Coronavirus Infections - virology
/ COVID-19
/ Genomes
/ Humans
/ Mice
/ Middle East respiratory syndrome
/ Nucleocapsid Proteins - immunology
/ Paraffin Embedding - methods
/ Pathology, Molecular - methods
/ Pneumonia, Viral - pathology
/ Proteins
/ Rabbits
/ RNA
/ RNA, Viral - isolation & purification
/ Severe acute respiratory syndrome coronavirus 2
/ Viruses
