Journal Article

Effects of Bacillus altitudinis inoculants on cigar tobacco leaf fermentation

Chen, Xi, Qiao, Jingyu, Wang, Zhi, Song, Wen, Yang, Jinpeng, Chen, Xiong, Yu, Jun

2024

Overview

Microbial succession and metabolic adjustment during cigar tobacco leaf (CTL) fermentation are key factors to improve the quality and flavor of CTLs. However, the interactions in the above processes remain to be further elucidated. inoculants were added to the CTLs, and metagenomics and metabolomics were used to analyze the effects of the inoculants on regulating microbial succession, metabolic shift, and aroma production during fermentation. The addition of the inoculants reinforced the CTL macromolecule transformation and facilitated the aroma production efficiently, and the total aroma production was increased by 43% compared with natural fermentation. The omics analysis showed that was a main contributor to fatty acid degradation, inositol phosphate metabolism, energy supply (oxidative phosphorylation), nutrient transport (ABC transporter and phosphotransferase system [PTS]), and aroma production (terpenoid backbone biosynthesis, phenylalanine metabolism, and degradation of aromatic compounds). Furthermore, was positively correlated with TCA cycle intermediates (citric acid, fumaric acid, and aconitic acid), cell wall components, peptidoglycan intermediates (GlcNAc-1-P and UDP-GlcNAc), and phytic acid degradation products (inositol). The characteristics collectively showed to be the most dominant in the microbial community at the genus level during microflora succession. The addition of the inoculants supplemented the nutritional components of the CTLs, enhanced the metabolic activity and diversity of bacteria such as , improved their competitive advantages in the microflora succession, and facilitated the richness of microbial communities. Additionally, a metabolic shift in nicotine degradation and NAD + anabolism from to in fermentation with inoculants was first observed. Meanwhile, the significantly correlative differential metabolites with and were a metabolic complement, thus forming a completely dynamic fermentation ecosystem. The results provided evidence for CTL fermentation optimization.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: ABC transporter / Aconitic acid / Aroma / aroma substance / Aromatic compounds / Bacillus altitudinis / Bacillus altitudinis inoculants / Bioengineering and Biotechnology / Cell walls / Cellulose / cigar tobacco leaf / Cigarette industry / Cigars / Citric acid / Corynebacterium / Cytotoxicity / Degradation products / Dietary supplements / Energy metabolism / Fermentation / Fumaric acid / Genetic transformation / Inositol phosphate / Intermediates / Leaves / Lymphocytes T / Metabolism / Metabolites / Metagenomics / metagenomics and metabolomics / Microbiomes / Microflora / Oxidative phosphorylation / Peptidoglycans / Phosphotransferase / Staphylococcus / Tobacco / Tricarboxylic acid cycle