Journal Article

Antimicrobial Activity and 70S Ribosome Binding of Apidaecin-Derived Api805 with Increased Bacterial Uptake Rate

Ludwig, Tobias, Krizsan, Andor, Hoffmann, Ralf, Mohammed, Gubran Khalil

2022

Overview

In view of the global spread of multiresistant bacteria and the occurrence of panresistant bacteria, there is an urgent need for antimicrobials with novel modes of action. A promising class is antimicrobial peptides (AMPs), including them proline-rich AMPs (PrAMPs), which target the 70S ribosome to inhibit protein translation. Here, we present a new designer peptide, Api805, combining the N- and C-terminal sequences of PrAMPs Api137 and drosocin, respectively. Api805 was similarly active against two Escherichia coli B strains but was inactive against E. coli K12 strain BW25113. These different activities could not be explained by the dissociation constants measured for 70S ribosome preparations from E. coli K12 and B strains. Mutations in the SbmA transporter that PrAMPs use to pass the inner membrane or proteolytic degradation of Api805 by lysate proteases could not explain this either. Interestingly, Api805 seems not to bind to the known binding sites of PrAMPs at the 70S ribosome and inhibited in vitro protein translation, independent of release factors, most likely using a “multimodal effect”. Interestingly, Api805 entered the E. coli B strain Rosetta faster and at larger quantities than the E. coli K-12 strain BW25113, which may be related to the different LPS core structure. In conclusion, slight structural changes in PrAMPs significantly altered their binding sites and mechanisms of action, allowing for the design of different antibiotic classes.

• Publisher: MDPI AG,MDPI
• Subject: Antibiotics / Antiinfectives and antibacterials / Antimicrobial activity / Antimicrobial agents / Antimicrobial peptides / apidaecin / Bacteria / bacterial ribosome / Binding sites / Biodegradation / Drug dosages / Drug resistance / E coli / Escherichia coli / Gram-positive bacteria / Mammals / Mutation / novel mode of action / Peptides / proline-rich antimicrobial peptide / Protein expression / Proteins / Proteolysis / Strains (organisms) / Termination factors / Translation