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CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans
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CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans
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Journal Article
CreA-independent carbon catabolite repression of cellulase genes by trimeric G-protein and protein kinase A in Aspergillus nidulans
2019
Overview
Cellulase production in filamentous fungi is repressed by various carbon sources. In our preliminary survey in
Aspergillus nidulans
, degree of de-repression differed depending on carbon sources in a mutant of
creA
, encoding the transcriptional repressor for carbon catabolite repression (CCR). To further understand mechanisms of CCR of cellulase production, we compared the effects of
creA
deletion with deletion of protein kinase A (
pkaA
) and G (
ganB
) genes, which constitute a nutrient sensing and signaling pathway. In plate culture with carboxymethyl cellulose and
d
-glucose, deletion of
pkaA
and
ganB
, but not
creA
, led to significant de-repression of cellulase production. In submerged culture with cellobiose and
d
-glucose or 2-deoxyglucose, both
creA
or
pkaA
single deletion led to partial de-repression of cellulase genes with the highest level by their double deletion, while
ganB
deletion caused de-repression comparable to that of the
creA
/
pkaA
double deletion. With ball-milled cellulose and
d
-glucose, partial de-repression was detected by deletion of
creA
but not of
pkaA
or
ganB
. The
creA
/
pkaA
or
creA
/
ganB
double deletion led to earlier expression than the
creA
deletion. Furthermore, the effect of each deletion with
d
-xylose or L-arabinose as the repressing carbon source was significantly different from that with
d
-glucose,
d
-fructose, and
d
-mannose. Consequently, this study revealed that PkaA and GanB participate in CreA-independent CCR and that contribution of CreA, PkaA, and GanB in CCR differs depending on the inducers, repressing carbon sources, and culture conditions (plate or submerged). Further study of CreA-independent mechanisms is needed to fully understand CCR in filamentous fungi.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
/ Aspergillus nidulans - genetics
/ Biomedical and Life Sciences
/ cAMP-dependent protein kinase
/ Carbon
/ Catabolite Repression - genetics
/ Cloning
/ Cyclic AMP-Dependent Protein Kinases - genetics
/ Cyclic GMP-Dependent Protein Kinases - genetics
/ Deletion
/ Enzymes
/ Fructose
/ Fungal Proteins - metabolism
/ Fungi
/ Gene Expression Regulation, Fungal
/ Genes
/ Glucose
/ GTP-Binding Proteins - genetics
/ Kinases
/ Mannose
/ Microbial Genetics and Genomics
/ mutants
/ Plasmids
/ Proteins
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ surveys
/ Xylose
