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Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing
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Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing
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Journal Article
Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing
2021
Overview
Introduction
Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process.
Material and methods
In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through
intI-attC
and 16S rRNA amplicons deep sequencing.
Results and discussion
ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to
fosB3
and
novA
were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance.
Conclusions
Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.
Publisher
Springer International Publishing,Springer Nature B.V
