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Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs
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Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs
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Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs
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Journal Article
Transcriptome and proteome characterization of surface ectoderm cells differentiated from human iPSCs
2016
Overview
Surface ectoderm (SE) cells give rise to structures including the epidermis and ectodermal associated appendages such as hair, eye and the mammary gland. In this study, we validate a protocol that utilizes BMP4 and the γ-secretase inhibitor DAPT to induce SE differentiation from human induced pluripotent stem cells (hiPSCs). hiPSC-differentiated SE cells expressed markers suggesting their commitment to the SE lineage. Computational analyses using integrated quantitative transcriptomic and proteomic profiling reveal that TGFβ superfamily signaling pathways are preferentially activated in SE cells compared with hiPSCs. SE differentiation can be enhanced by selectively blocking TGFβ-RI signaling. We also show that SE cells and neural ectoderm cells possess distinct gene expression patterns and signaling networks as indicated by functional Ingenuity Pathway Analysis. Our findings advance current understanding of early human SE cell development and pave the way for modeling of SE-derived tissue development, studying disease pathogenesis and development of regenerative medicine approaches.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/100
/ 14/63
/ 631/532
/ 692/4017
/ 82
/ 82/58
/ 82/80
/ Bone Morphogenetic Protein 4 - pharmacology
/ Cell Differentiation - drug effects
/ Ectoderm
/ Eye
/ Gene Expression Profiling - methods
/ Gene Expression Regulation - drug effects
/ Gene Regulatory Networks - drug effects
/ Hair
/ Humanities and Social Sciences
/ Humans
/ Induced Pluripotent Stem Cells - cytology
/ Induced Pluripotent Stem Cells - drug effects
/ Induced Pluripotent Stem Cells - metabolism
/ Inhibitory postsynaptic potentials
/ Science
/ Signal Transduction - drug effects
