Journal Article

Crystal Violet and XTT Assays on Staphylococcus aureus Biofilm Quantification

Li, Lin, Liang, Yanrui, Lin, Shiqi, Deng, Yang, Xu, Zhenbo, Li, Bing, Chen, Dingqiang

2016

Overview

Staphylococcus aureus ( S. Aureus ) is a common food-borne pathogenic microorganism. Biofilm formation remains the major obstruction for bacterial elimination. The study aims at providing a basis for determining S. aureus biofilm formation. 257 clinical samples of S. aureus isolates were identified by routine analysis and multiplex PCR detection and found to contain 227 MRSA, 16 MSSA, 11 MRCNS, and 3 MSCNS strains. Two assays for quantification of S. aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 %). However, most isolates in XTT assay showed weak metabolic activity (77.0 %), while the rest showed moderate metabolic activity (17.9 %) or high metabolic activity (5.1 %). In this study, we found a distinct strain-to-strain dissimilarity in terms of both biomass formation and metabolic activity, and it was concluded from this study that two assays were mutual complementation rather than being comparison.

• Publisher: Springer US,Springer Nature B.V
• Subject: biofilm / Biofilms / biomass / Biomedical and Life Sciences / Biotechnology / Food contamination & poisoning / gentian violet / Gentian Violet - chemistry / Humans / Life Sciences / methicillin-resistant Staphylococcus aureus / Microbiology / Microorganisms / polymerase chain reaction / Staining and Labeling - methods / Staphylococcal Infections - microbiology / Staphylococcus aureus / Staphylococcus aureus - chemistry / Staphylococcus aureus - genetics / Staphylococcus aureus - isolation & purification / Staphylococcus aureus - physiology / Staphylococcus infections / tetrazolium / Tetrazolium Salts - chemistry