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Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media
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Journal Article
Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media
2015
Overview
Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in media supplemented with either human platelet lysates (HPL), serum-free media/xeno-free FDA-approved culture medium (SFM/XF), or fetal bovine serum (FBS) and the effects on their properties were investigated. The immunophenotype of resting and IFN-γ primed BMMSC and AdMSC remained unaltered in all media. Both HPL and SFM/XF increased the proliferation of BMMSC and AdMSC. Expansion of BMMSC and AdMSC in HPL increased their differentiation, compared to SFM/XF and FBS. Resting BMMSC and AdMSC, expanded in FBS or SFM/XF, demonstrated potent immunosuppressive properties in both non-primed and IFN-γ primed conditions, whereas HPL-expanded MSC exhibited diminished immunosuppressive properties. Finally, IFN-γ primed BMMSC and AdMSC expanded in SFM/XF and HPL expressed attenuated levels of IDO-1 compared to FBS. Herein, we provide strong evidence supporting the use of the FDA-approved SFM/XF medium, in contrast to the HPL medium, for the expansion of MSC towards therapeutic applications.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/106
/ 13/31
/ 13/51
/ 692/4020
/ Alkaline Phosphatase - genetics
/ Animals
/ Cattle
/ Cell Culture Techniques - methods
/ Cell Differentiation - drug effects
/ Cell Differentiation - genetics
/ Cell Extracts - pharmacology
/ Cell Proliferation - drug effects
/ Cell Proliferation - genetics
/ Culture Media - pharmacology
/ Culture Media, Serum-Free - pharmacology
/ Gene Expression - drug effects
/ Humanities and Social Sciences
/ Humans
/ Indoleamine-Pyrrole 2,3,-Dioxygenase - metabolism
/ Interferon-gamma - pharmacology
/ Lysates
/ Mesenchymal Stromal Cells - cytology
/ Mesenchymal Stromal Cells - drug effects
/ Mesenchymal Stromal Cells - metabolism
/ Reverse Transcriptase Polymerase Chain Reaction
/ Science
