Journal Article

MiR-21-5p but not miR-1-3p expression is modulated by preconditioning in a rat model of myocardial infarction

Raupach, Annika, Niestegge, Julia, Bauer, Inge, Brandenburger, Timo, Klemm-Meyer, Swantje, Huhn, Ragnar, Torregroza, Carolin, Grievink, Hilbert, Feige, Katharina, Heinen, André

2020

Overview

Isoflurane (Iso) preconditioning (PC) is known to be cardioprotective against ischemia/reperfusion (I/R) injury. It was previously shown that microRNA-21-5p (miR-21-5p) is regulated by Iso-PC. It is unclear, if expression of cardiac enriched miR-1-3p is also affected by Iso-PC, and associated with activation of HIF1α (hypoxia-inducible factor 1-alpha).  Male Wistar rats (n = 6–8) were randomly assigned to treatment with or without 1 MAC Iso for 30 min, followed by 25 min of regional myocardial ischemia, with 120 min reperfusion. At the end of reperfusion, myocardial expression of miR-1-3p, miR-21-5p and mRNAs of two HIF-1α-dependent genes, VEGF (vascular endothelial growth factor) and HO-1 (heme oxygenase-1), were determined by quantitative PCR. Protein expression of a miR-21 target gene, PDCD4 (programmed cell death protein 4), was assessed by western blot analysis. Infarct sizes were analyzed with triphenyltetrazoliumchloride staining. MiR-21-5p expression was increased by Iso, whereas expression of miR-1-3p was not altered. The expression of VEGF but not HO-1 was induced by Iso. Iso-PC reduced infarct sizes compared to untreated controls. No regulation of miRNA and mRNA expression was detected after I/R. PDCD4 protein expression was not affected after Iso exposure. Expression of miR-21-5p, in contrast to miR-1-3p, is altered during this early time point of Iso-PC. HIF1α signaling seems to be involved in miR-21-5p regulation.

• Publisher: Springer Netherlands,Springer Nature B.V
• Subject: Animal Anatomy / Animal Biochemistry / Animal models / Apoptosis / Biomedical and Life Sciences / Cell death / Gene expression / genes / Heme / heme oxygenase (biliverdin-producing) / Heme oxygenase (decyclizing) / Histology / Hypoxia-inducible factor 1 / Hypoxia-inducible factor 1a / infarction / Ischemia / Isoflurane / Life Sciences / males / microRNA / MicroRNAs / miRNA / Morphology / Myocardial infarction / Myocardial ischemia / Original / Original Article / Oxygenase / programmed cell death / Protein expression / protein synthesis / Proteins / quantitative polymerase chain reaction / Reperfusion / Rodents / Vascular endothelial growth factor / vascular endothelial growth factors / Western blotting