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Recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells
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Recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells
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Recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells
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Journal Article
Recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells
2016
Overview
•A recombinant BCG expressing Ag85B-IL-7 fusion protein was constructed.•IL-17A+ γδ T and Th1 cells were increased in mice inoculated with rBCG-Ag85B-IL-7.•Th1 cells were impaired in rBCG inoculated mice lacking IL-17A+ γδ T cells.•IL-17A+ γδ T cells subsequently augment the Th1 response to BCG.
Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette–Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacterial lysate of rBCG-Ag85B-IL-7. rBCG-Ag85B-IL-7 was the same in number as control rBCG expressing Ag85B (rBCG-Ag85B) in the lung at the early stage after intravenous inoculation, whereas the numbers of IL-17A+ γδ T cells and Ag-specific Th1 cells were significantly higher in the lungs of mice inoculated with rBCG-Ag85B-IL-7 than those inoculated with rBCG-Ag85B. The Ag-specific Th1 cell response was impaired in mice lacking IL-17A+ γδ T cells after inoculation with rBCG-Ag85B-IL-7. Thus, rBCG-Ag85B-IL-7 increases the pool size of IL-17A+ γδ T cells, which subsequently augment the Th1 response to mycobacterial infection.
Publisher
Elsevier Ltd
Subject
/ Acyltransferases - immunology
/ Animals
/ antigens
/ Antigens, Bacterial - genetics
/ Antigens, Bacterial - immunology
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - immunology
/ BCG
/ IL-17A
/ IL-7
/ lungs
/ Mice
/ Mycobacterium bovis - genetics
/ Mycobacterium bovis - growth & development
/ Mycobacterium bovis - immunology
/ Receptors, Antigen, T-Cell, gamma-delta - immunology
/ Recombinant Fusion Proteins - immunology
/ T-Lymphocyte Subsets - immunology
/ vaccines
