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Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix
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Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix
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Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix
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Journal Article
Differentiation of human embryonic stem cells to hepatocyte-like cells on a new developed xeno-free extracellular matrix
2014
Overview
Human embryonic stem cells (hESCs) provide a new source for hepatocyte production in translational medicine and cell replacement therapy. The reported hESC-derived hepatocyte-like cells (HLCs) were commonly generated on Matrigel, a mouse cell line-derived extracellular matrix (ECM). Here, we performed the hepatic lineage differentiation of hESCs following a stepwise application of growth factors on a newly developed serum- and xeno-free, simple and cost-benefit ECM, designated “RoGel,” which generated from a modified conditioned medium of human fibroblasts. In comparison with Matrigel, the differentiated HLCs on both ECMs expressed similar levels of hepatocyte-specific genes, secreted α-fetoprotein, and metabolized ammonia, showed glycogen storage activity as well as low-density lipoprotein and indocyanine green uptake. The transplantation of hESC–HLCs into the carbon tetrachloride-injured liver demonstrated incorporation of the cells into the host mouse liver and the expression of albumin. The results suggest that the xeno-free and cost-benefit matrix may be applicable in bioartificial livers and also may facilitating a clinical application of human pluripotent stem cell-derived hepatocytes in the future.
Publisher
Springer Berlin Heidelberg,Springer Nature B.V
Subject
alpha-Fetoproteins - biosynthesis
/ alpha-Fetoproteins - secretion
/ Animals
/ Biomedical and Life Sciences
/ Collagen
/ Embryonic Stem Cells - cytology
/ Embryonic Stem Cells - transplantation
/ Embryos
/ GATA4 Transcription Factor - biosynthesis
/ Goosecoid Protein - biosynthesis
/ Hepatocyte Nuclear Factor 3-beta - biosynthesis
/ Humans
/ Indocyanine Green - metabolism
/ Induced Pluripotent Stem Cells - cytology
/ Induced Pluripotent Stem Cells - transplantation
/ Laminin
/ Lipoproteins, LDL - metabolism
/ Matrix
/ Mice
/ SOXB1 Transcription Factors - biosynthesis
