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The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions

by Fagunloye, Adeola A , Malc, Ewa P , Bonilla, Braulio , Roberts, Steven A , Brown, Alexander J , Hengel, Sarah R , Mitchell, Debra , Zaher, Hani S , Luong, Thong T , Mertz, Tony M , Russell, Reagan A , Mosammaparast, Nima , Mieczkowski, Piotr A , Vyas, Rudri K , Bernstein, Kara A , Rapchak, Kyle S , Pressimone, Catherine A

in Adducts / alkyation damage / Alkylation / Biochemistry and Chemical Biology / Cell Biology / Cytosine / Cytotoxicity / DNA biosynthesis / DNA damage / DNA repair / Ectopic expression / Enzymes / Genomes / Homologous recombination / Homologous Recombination - drug effects / Lesions / Methyl methanesulfonate / Methyl Methanesulfonate - pharmacology / Mutagenesis / Mutagens - pharmacology / Mutation / Rad51 / Rad51 paralogs / Saccharomyces cerevisiae - drug effects / Saccharomyces cerevisiae - genetics / Saccharomyces cerevisiae - metabolism / Saccharomyces cerevisiae Proteins - metabolism / Shu complex / Single-stranded DNA / Transcription / Yeast

2021

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The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions

2021

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2021

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Journal Article

The Shu complex prevents mutagenesis and cytotoxicity of single-strand specific alkylation lesions

Fagunloye, Adeola A,

Malc, Ewa P,

Bonilla, Braulio,

Roberts, Steven A,

Brown, Alexander J,

Hengel, Sarah R,

Mitchell, Debra,

Zaher, Hani S,

Luong, Thong T,

Mertz, Tony M,

Russell, Reagan A,

Mosammaparast, Nima,

Mieczkowski, Piotr A,

Vyas, Rudri K,

Bernstein, Kara A,

Rapchak, Kyle S,

Pressimone, Catherine A

2021

Overview

Three-methyl cytosine (3meC) are toxic DNA lesions, blocking base pairing. Bacteria and humans express members of the AlkB enzymes family, which directly remove 3meC. However, other organisms, including budding yeast, lack this class of enzymes. It remains an unanswered evolutionary question as to how yeast repairs 3meC, particularly in single-stranded DNA. The yeast Shu complex, a conserved homologous recombination factor, aids in preventing replication-associated mutagenesis from DNA base damaging agents such as methyl methanesulfonate (MMS). We found that MMS-treated Shu complex-deficient cells exhibit a genome-wide increase in A:T and G:C substitutions mutations. The G:C substitutions displayed transcriptional and replicational asymmetries consistent with mutations resulting from 3meC. Ectopic expression of a human AlkB homolog in Shu-deficient yeast rescues MMS-induced growth defects and increased mutagenesis. Thus, our work identifies a novel homologous recombination-based mechanism mediated by the Shu complex for coping with alkylation adducts.

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Publisher

eLife Sciences Publications Ltd,eLife Sciences Publications, Ltd

Subject

Adducts

/ alkyation damage

/ Alkylation

/ Biochemistry and Chemical Biology

/ Cell Biology

/ Cytosine

/ Cytotoxicity