Asset Details
Do you wish to reserve the book?
Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles
Do you wish to request the book?
Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles
2023
Overview
Novel rapid methodologies for the detection of bacteria have been recently investigated and applied. In hospital environments, infections by pathogens are very common and can cause serious health problems. Pseudomonas aeruginosa is one of the most common bacteria, which can grow in hospital equipment such as catheters and respirators. Even at low concentrations, it can cause severe infections as it is resistant to antibiotics and other treatments. Based on this subject’s relevance, this work aimed to develop a colorimetric biosensor using aptamer-functionalized gold nanoparticles for identifying P. aeruginosa. The detection mechanism is based on the color change of gold nanoparticles (AuNPs) from red to blue-purple through NaCl induction after bacteria incubation and aptamer-target binding. First, AuNPs were synthesized and characterized. The influence of aptamer and sodium chloride concentration on the agglomeration of AuNPs was investigated. Optimization of aptamer concentration and salt addition were performed. The best condition for detection was 5 µM aptamers and 200 mM of NaCl. In this case, P. aeruginosa was detected after 5 h for concentrations from 108 to 105 CFU mL−1, being 105 and 104 CFU mL−1 the detection limit for color change by the naked eye and UV–Vis spectrometry, respectively. In addition, other bacteria such as E. coli, S. typhimurium, and Enterobacteriaceae bacterium were also detected with color changing from red to gray. Finally, it was confirmed that the salt incubation time can be 2 h, and that the ideal aptamer concentration is 5 µM. Thus, the colorimetric analysis can be a simple and fast detection method for P. aeruginosa in the range of 108 to 105 CFU mL−1 to the naked eye.Key Points• A new method for rapid detection of Pseudomonas aeruginosa• Aptamers conjugated with gold nanoparticles allow pathogen detection by colorimetry• No need for previous surface modification of nanoparticles
Publisher
Springer Nature B.V
Subject
