Asset Details
Do you wish to reserve the book?
Angiotensin II regulates the LARG/RhoA/MYPT1 axis in rat vascular smooth muscle in vitro
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Angiotensin II regulates the LARG/RhoA/MYPT1 axis in rat vascular smooth muscle in vitro
Do you wish to request the book?
Angiotensin II regulates the LARG/RhoA/MYPT1 axis in rat vascular smooth muscle in vitro
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Angiotensin II regulates the LARG/RhoA/MYPT1 axis in rat vascular smooth muscle in vitro
2012
Overview
Aim： To identify a key protein that binds monomeric G protein RhoA and activates the RhoA/Rho kinase/MYPT1 axis in vascular smooth muscle cells （VSMCs） upon angiotensin II （Ang ｜｜） stimulation. Methods： Primary cultured VSMCs from Sprague-Dawley rats were transfected with siRNAs against leukemia-associated RhoGEF （LARG）, and then treated with Ang ｜｜, Iosartan, PD123319, or ValS-Ang ｜｜. The target mRNA and protein levels were determined using qPCR and Western blot analysis, respectively. Rat aortic rings were isolated, and the isometric contraction was measured with a force transducer and recorder. Results： Stimulation with Ang ｜｜ （0.1 μmol/L） for 0.5 h significantly increased the level of LARG mRNA in VSMCs. At 3, 6, and 9 h after the treatment with Ang ｜｜ （0.1μmol/L） plus AT2 antagonist PD123319 （1 μmol/L） or with AT1 agonist ValS-Ang II （1 μmol/L）, the LARG protein, RhoA activity, and phosphorylation level of myosin phosphatase target subunit 1 （MYPT1） in VSMCs were significantly increased. Knockdown of LARG with siRNA reduced these effects caused by AT1 receptor activation. In rat aortic rings pretreated with LARG siRNA, Ang II-induced contraction was diminished. Conclusion： Ang II upregulates LARG gene expression and activates the LARG/RhoA/MYPT1 axis via ATe, thereby maintaining vascular tone.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Angiotensin II - pharmacology
/ Animals
/ Aorta, Thoracic - drug effects
/ Aorta, Thoracic - physiopathology
/ Biomedical and Life Sciences
/ Gene Expression Regulation - drug effects
/ Guanine Nucleotide Exchange Factors - genetics
/ Isometric Contraction - drug effects
/ Male
/ Muscle, Smooth, Vascular - drug effects
/ Muscle, Smooth, Vascular - enzymology
/ Muscle, Smooth, Vascular - metabolism
/ Original
/ Protein Phosphatase 1 - metabolism
/ Rats
/ Real-Time Polymerase Chain Reaction
/ Rho Guanine Nucleotide Exchange Factors
/ rhoA GTP-Binding Protein - metabolism
/ RNA, Small Interfering - genetics
/ SD大鼠
/ siRNA
/ Vaccine
/ 体外
/ 受体激动剂
/ 蛋白磷酸酶
/ 血管平滑肌细胞
/ 血管紧张素
/ 轴
