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Engineering herbicide resistance via prime editing in rice
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Engineering herbicide resistance via prime editing in rice
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Journal Article
Engineering herbicide resistance via prime editing in rice
2020
Overview
In plants, homology‐directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence (Ali et al., 2020; Butt et al., 2017). [...]cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence and the possibility of off‐target mutations at other loci (Rees and Liu, 2018). By these mutations, PvUII site was lost and two sites Pst1 and BbsI were generated. (f) The PvUII enriched DNA samples are confirmed by Sanger sequencing. (g) Schematic of rice locus TB1 (TEOSINTE BRANCHED1). The OsIPA transcription factor reduces the number of unproductive tillers and improves rice yield. [...]we targeted rice TEOSINTE BRANCHED 1 (OsTB1), a member of the TEOSINTE BRANCHED1, CYCLOIDEA AND PCF TRANSCRIPTION FACTOR gene family (Figure 1g).
