Journal Article

Lewis y Regulate Cell Cycle Related Factors in Ovarian Carcinoma Cell RMG-I in Vitro via ERK and Akt Signaling Pathways

Zhang, Shulan, Iwamori, Masao, Liu, Juanjuan, Lin, Bei, Liu, Qing, Hao, Yingying, Liu, Dawo, Liu, Shuice, Hou, Rui

2012

Overview

Objective: To investigate the effect of Lewis y overexpression on the expression of proliferation-related factors in ovarian cancer cells. Methods: mRNA levels of cyclins, CDKs, and CKIs were measured in cells before and after transfection with the α1,2-fucosyltransferase gene by real-time PCR, and protein levels of cyclins, CDKs and CKIs were determined in cells before and after gene transfection by Western blot. Results: Lewis y overexpression led to an increase in both mRNA and protein expression levels of cyclin A, cyclin D1 and cyclin E in ovarian cancer cells, decrease in both mRNA and protein expression levels of p16 and p21, and decrease of p27 at only the protein expression level without change in its mRNA level. There were no differences in proteins and the mRNA levels of CDK2, CDK4 and CDK6 before and after gene transfection. Anti-Lewis y antibody, ERK and PI3K pathway inhibitors PD98059 and LY294002 reduced the difference in cyclin and CKI expression caused by Lewis y overexpression. Conclusion: Lewis y regulates the expression of cell cycle-related factors through ERK/MAPK and PI3K/Akt signaling pathways to promote cell proliferation.

• Publisher: MDPI AG,Molecular Diversity Preservation International (MDPI)
• Subject: 1-Phosphatidylinositol 3-kinase / AKT protein / Antibodies / Antibodies - immunology / Apoptosis / Cell cycle / Cell division / Cell growth / Cell Line, Tumor / Cell proliferation / Chromones - pharmacology / Cyclin A / Cyclin A - genetics / Cyclin A - metabolism / cyclin D1 / Cyclin D1 - genetics / Cyclin D1 - metabolism / Cyclin E / Cyclin E - genetics / Cyclin E - metabolism / Cyclin-dependent kinase / Cyclin-Dependent Kinase 2 - genetics / Cyclin-Dependent Kinase 2 - metabolism / Cyclin-dependent kinase 4 / Cyclin-Dependent Kinase 4 - genetics / Cyclin-Dependent Kinase 4 - metabolism / Cyclin-Dependent Kinase 6 - genetics / Cyclin-Dependent Kinase 6 - metabolism / Cyclin-Dependent Kinase Inhibitor p16 - genetics / Cyclin-Dependent Kinase Inhibitor p16 - metabolism / Cyclin-Dependent Kinase Inhibitor p21 - genetics / Cyclin-Dependent Kinase Inhibitor p21 - metabolism / Cyclin-Dependent Kinase Inhibitor p27 - genetics / Cyclin-Dependent Kinase Inhibitor p27 - metabolism / Cyclin-dependent kinases / Extracellular signal-regulated kinase / Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors / Extracellular Signal-Regulated MAP Kinases - metabolism / Female / Flavonoids - pharmacology / Fucosyltransferases - genetics / Fucosyltransferases - metabolism / Galactoside 2- alpha -L-fucosyltransferase / Gene expression / Humans / Kinases / Lewis Blood-Group System - genetics / Lewis Blood-Group System - immunology / Lewis Blood-Group System - metabolism / MAP kinase / Morpholines - pharmacology / mRNA / Ovarian cancer / Ovarian Neoplasms - metabolism / Ovarian Neoplasms - pathology / Polymerase chain reaction / Protein expression / Proteins / Proto-Oncogene Proteins c-akt - metabolism / RNA, Messenger - metabolism / Signal transduction / Signal Transduction - drug effects / Transfection / Western blotting