Journal Article

Effects of plant sterol esters in skimmed milk and vegetable-fat-enriched milk on serum lipids and non-cholesterol sterols in hypercholesterolaemic subjects: a randomised, placebo-controlled, crossover study

Serra, Mercè, Casas-Agustench, Patricia, Pérez-Heras, Ana, Pintó, Xavier, Ros, Emilio, Cofán, Montserrat, Trautwein, Elke A.

2012

Overview

Plant sterol (PS)-supplemented foods are recommended to help in lowering serum LDL-cholesterol (LDL-C). Few studies have examined the efficacy of PS-enriched skimmed milk (SM) or semi-SM enriched with vegetable fat (PS-VFM). There is also insufficient information on factors predictive of LDL-C responses to PS. We examined the effects of PS-SM (0·1 % dairy fat) and PS-VFM (0·1 % dairy fat plus 1·5 % vegetable fat) on serum lipids and non-cholesterol sterols in hypercholesterolaemic individuals. In a placebo-controlled, crossover study, forty-three subjects with LDL-C>1300 mg/l were randomly assigned to three 4-week treatment periods: control SM, PS-SM and PS-VFM, with 500 ml milk with or without 3·4 g PS esters (2 g free PS). Serum concentrations of lipids and non-cholesterol sterols were measured. Compared to control, LDL-C decreased by 8·0 and 7·4 % (P < 0·015, both) in the PS-SM and PS-VFM periods, respectively. Serum lathosterol:cholesterol (C) ratios increased by 11–25 %, while sitosterol:C and campesterol:C ratios increased by 70–120 % with both the PS-fortified milk. Adjusted LDL-C reductions were variably enhanced in participants with basal low serum lathosterol/C or conversely high sitosterol/C and campesterol/C. Subjects with post-treatment serum PS:C ratios above the median showed mean LDL-C changes of − 5·9 to − 10·4 %, compared with 1·7 to − 2·9 % below the median. In conclusion, consumption of 2 g/d of PS as PS-SM and PS-VFM lowered LDL-C in hypercholesterolaemic subjects to a similar extent. Basal and post-treatment changes in markers of cholesterol metabolism indicating low cholesterol synthesis and high cholesterol absorption predicted improved LDL-C responses to PS.

• Publisher: Cambridge University Press
• Subject: absorption / Adult / Animals / Anticholesteremic Agents - pharmacology / Anticholesteremic Agents - therapeutic use / Biological and medical sciences / blood lipids / blood serum / campesterol / Cholesterol / cholesterol metabolism / Cholesterol, LDL - blood / Cross-Over Studies / Dietary Fats - pharmacology / Dietary Fats - therapeutic use / Dietary Supplements / Disorders of blood lipids. Hyperlipoproteinemia / Esters / Esters - pharmacology / Esters - therapeutic use / Feeding. Feeding behavior / Female / Food, Fortified / Fundamental and applied biological sciences. Psychology / Humans / hypercholesterolemia / Hypercholesterolemia - blood / Hypercholesterolemia - diet therapy / Lipids / Low density lipoprotein / low density lipoprotein cholesterol / Male / Medical sciences / Metabolic diseases / Metabolism / Metabolism and Metabolic Studies / Middle Aged / Milk / Phytosterols - pharmacology / Phytosterols - therapeutic use / Phytotherapy / Plant Extracts - pharmacology / Plant Extracts - therapeutic use / Plant Oils - pharmacology / Plant Oils - therapeutic use / skim milk / sterol esters / Sterols / Sterols - blood / Vegetables / Vertebrates: anatomy and physiology, studies on body, several organs or systems