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Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
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Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
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Journal Article
Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells
2019
Overview
Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.
