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Bio-enzymes for inhibition and elimination of Escherichia coli O157:H7 biofilm and their synergistic effect with sodium hypochlorite
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Bio-enzymes for inhibition and elimination of Escherichia coli O157:H7 biofilm and their synergistic effect with sodium hypochlorite
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Journal Article
Bio-enzymes for inhibition and elimination of Escherichia coli O157:H7 biofilm and their synergistic effect with sodium hypochlorite
2019
Overview
Escherichia coli
O157:H7 is one of the most important pathogens worldwide. In this study, three different kinds of enzymes, DNase I, proteinase K and cellulase were evaluated for inhibitory or degrading activity against
E. coli
O157:H7 biofilm by targeting extracellular DNA, proteins, and cellulose, respectively. The cell number of biofilms formed under proteinase K resulted in a 2.43 log CFU/cm
2
reduction with an additional synergistic 3.72 log CFU/cm
2
reduction after NaClO post-treatment, while no significant reduction occurred with NaClO treatment alone. It suggests that protein degradation could be a good way to control the biofilm effectively. In preformed biofilms, all enzymes showed a significant reduction of 16.4–36.7% in biofilm matrix in 10-fold diluted media (
p
< 0.05). The sequential treatment with proteinase K, cellulase, and NaClO showed a significantly higher synergistic inactivation of 2.83 log CFU/cm
2
compared to 1.58 log CFU/cm
2
in the sequence of cellulase, proteinase K, and NaClO (
p
< 0.05). It suggests that the sequence of multiple enzymes can make a significant difference in the susceptibility of biofilms to NaClO. This study indicates that the combination of extracellular polymeric substance-degrading enzymes with NaClO could be useful for the efficient control of
E. coli
O157:H7 biofilms.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/28
/ 14/34
/ Biofilms
/ Biofilms - growth & development
/ Deoxyribonuclease I - metabolism
/ E coli
/ Endopeptidase K - metabolism
/ Enzymes
/ Escherichia coli O157 - drug effects
/ Escherichia coli O157 - growth & development
/ Escherichia coli O157 - metabolism
/ Humanities and Social Sciences
/ Science
/ Sodium
