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Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1
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Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1
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Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1
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Journal Article
Identification of mesothelioma-specific sialylated epitope recognized with monoclonal antibody SKM9-2 in a mucin-like membrane protein HEG1
2018
Overview
The anti-mesothelioma mAb SKM9-2 recognizes the sialylated protein HEG homolog 1 (HEG1). HEG1 is a 400 kDa mucin-like membrane protein found on mesothelioma. SKM9-2 can detect mesothelioma more specifically and sensitively than other antibodies against current mesothelioma markers; therefore, SKM9-2 would be likely useful for the precise detection and diagnosis of malignant mesothelioma. In the present study, we investigated the epitope of SKM9-2. We analyzed the binding of SKM9-2 to truncated HEG1 and candidate epitope-fused glycosylphosphatidylinositol-anchor proteins. The epitope of SKM9-2 was identified as an
O
-glycosylated region, 893-SKSPSLVSLPT-903, in HEG1. An alanine scanning assay of the epitope showed that SKM9-2 bound to a simple epitope in HEG1, and the SKxPSxVS sequence within the epitope was essential for SKM9-2 recognition. Mass spectrometry analysis and lectin binding analysis of soluble epitope peptides indicated that the SKM9-2 epitope, in which Ser
897
was not glycosylated, contained two disialylated core 1
O
-linked glycan-modified serine residues, Ser
893
and Ser
900
. Neuraminidase treatment analysis also confirmed that the epitope in mesothelioma cells contained a similar glycan modification. The specific detection of mesothelioma with SKM9-2 can thus be performed by the recognition of sialylated glycan modification in the specific region of HEG1.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 82/103
/ 82/16
/ 82/29
/ 82/58
/ Alanine
/ Antibodies, Monoclonal - genetics
/ Antibodies, Monoclonal - immunology
/ Enzyme-Linked Immunosorbent Assay
/ Epitopes
/ Glycosylphosphatidylinositol
/ Humanities and Social Sciences
/ Humans
/ Membrane Proteins - genetics
/ Membrane Proteins - immunology
/ Mucin
/ N-Acetylneuraminic Acid - metabolism
/ Peptides
/ Polysaccharides - metabolism
/ Protein Binding - immunology
/ Proteins
/ Science
/ Serine
