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Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells
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Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells
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Journal Article
Surface functionalization of electrospun scaffolds using recombinant human decorin attracts circulating endothelial progenitor cells
2018
Overview
Decorin (DCN) is an important small leucine-rich proteoglycan present in the extracellular matrix (ECM) of many organs and tissues. Endothelial progenitor cells (EPCs) are able to interact with the surrounding ECM and bind to molecules such as DCN. Here, we recombinantly produced full-length human DCN under good laboratory practice (GLP) conditions, and after detailed immunological characterization, we investigated its potential to attract murine and human EPCs (mEPCs and hECFCs). Electrospun polymeric scaffolds were coated with DCN or stromal cell-derived factor-1 (SDF-1α) and were then dynamically cultured with both cell types. Cell viability was assessed via imaging flow cytometry. The number of captured cells was counted and compared with the non-coated controls. To characterize cell-scaffold interactions, immunofluorescence staining and scanning electron microscopy analyses were performed. We identified that DCN reduced T cell responses and attracted innate immune cells, which are responsible for ECM remodeling. A significantly higher number of EPCs attached on DCN- and SDF-1α-coated scaffolds, when compared with the uncoated controls. Interestingly, DCN showed a higher attractant effect on hECFCs than SDF-1α. Here, we successfully demonstrated DCN as promising EPC-attracting coating, which is particularily interesting when aiming to generate off-the-shelf biomaterials with the potential of
in vivo
cell seeding.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/100
/ 13/106
/ 13/107
/ 13/31
/ 14/63
/ 82/80
/ Animals
/ Cell Survival - drug effects
/ Decorin
/ Endothelial Progenitor Cells - drug effects
/ Endothelial Progenitor Cells - metabolism
/ Endothelial Progenitor Cells - ultrastructure
/ Extracellular Matrix - metabolism
/ Fluorescent Antibody Technique
/ Heart
/ Humanities and Social Sciences
/ Humans
/ Immunity
/ Leucine
/ Proteins
/ Recombinant Proteins - metabolism
/ Recombinant Proteins - pharmacology
/ Scanning electron microscopy
/ Science
