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Regeneration associated transcriptional signature of retinal microglia and macrophages
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Regeneration associated transcriptional signature of retinal microglia and macrophages
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Journal Article
Regeneration associated transcriptional signature of retinal microglia and macrophages
2019
Overview
Zebrafish have the remarkable capacity to regenerate retinal neurons following a variety of damage paradigms. Following initial tissue insult and a period of cell death, a proliferative phase ensues that generates neuronal progenitors, which ultimately regenerate damaged neurons. Recent work has revealed that Müller glia are the source of regenerated neurons in zebrafish. However, the roles of another important class of glia present in the retina, microglia, during this regenerative phase remain elusive. Here, we examine retinal tissue and perform QuantSeq. 3′mRNA sequencing/transcriptome analysis to reveal localization and putative functions, respectively, of
mpeg1
expressing cells (microglia/macrophages) during Müller glia-mediated regeneration, corresponding to a time of progenitor proliferation and production of new neurons. Our results indicate that in this regenerative state,
mpeg1
-expressing cells are located in regions containing regenerative Müller glia and are likely engaged in active vesicle trafficking. Further,
mpeg1
+ cells congregate at and around the optic nerve head. Our transcriptome analysis reveals several novel genes not previously described in microglia. This dataset represents the first report, to our knowledge, to use RNA sequencing to probe the microglial transcriptome in such context, and therefore provides a resource towards understanding microglia/macrophage function during successful retinal (and central nervous tissue) regeneration.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/19
/ 38
/ 38/91
/ 64
/ 64/116
/ Animals
/ Animals, Genetically Modified
/ Cell Differentiation - physiology
/ Ependymoglial Cells - cytology
/ Humanities and Social Sciences
/ Membrane Proteins - genetics
/ Membrane Proteins - metabolism
/ Nerve Regeneration - physiology
/ Neural Stem Cells - cytology
/ Neurons
/ Retina
/ Retinal Neurons - physiology
/ Science
