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Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR
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Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR
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Journal Article
Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR
2020
Overview
In this study, we measured the human epidermal growth factor receptor 2 (
HER2
) copy number in both tissue and plasma samples of gastric cancer patients by using droplet digital polymerase chain reaction (ddPCR) method. Eighty gastric cancer patients were enrolled and both formalin-fixed and paraffin-embedded tissue and preoperative plasma samples were collected. HER2 status was determined by HER2 immunohistochemistry (IHC)/silver
in situ
hybridization (SISH) in tissue samples and ddPCR of the target gene
HER2
and the reference gene eukaryotic translation initiation factor 2C, 1 in both tissue and plasma. The concordance rate of tissue HER2 status determined by IHC/SISH and
HER2
ddPCR was 90.0% (72/80), and the sensitivity and specificity of tissue ddPCR were 85.0% and 95.0%, respectively. The concordance rate of plasma ddPCR and IHC/SISH was 63.8% (51/80). The sensitivity, specificity, positive predictive value, and negative predictive value of plasma
HER2
ddPCR were 37.5%, 90.0%, 79.0%, and 59.0%, respectively. As
HER2
measurement by tissue ddPCR showed a high concordance rate with HER2 status by IHC/SISH, it could replace tissue IHC/SISH testing in gastric cancer. These findings may contribute to the development of tissue and plasma HER2 testing that would be useful in daily practice.
Publisher
Nature Publishing Group UK,Nature Publishing Group
