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Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
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Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
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Journal Article
Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
2016
Overview
The normal distribution of nuclear envelope transmembrane proteins (NETs) is disrupted in several human diseases. NETs are synthesized on the endoplasmic reticulum and then transported from the outer nuclear membrane (ONM) to the inner nuclear membrane (INM). Quantitative determination of the distribution of NETs on the ONM and INM is limited in available approaches, which moreover provide no information about translocation rates in the two membranes. Here we demonstrate a single-point single-molecule FRAP microscopy technique that enables determination of distribution and translocation rates for NETs
in vivo
.
Nuclear envelope transmembrane proteins (NETs) can reside in the outer or inner nuclear membrane, but distinguishing which membrane they reside in, and their translocation rate, is technically challenging. Here the authors develop a FRAP-based super-resolution microscopy method to obtain this information for several NETs.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/35
/ 14/63
/ Endoplasmic Reticulum - metabolism
/ Fluorescence Recovery After Photobleaching - instrumentation
/ Fluorescence Recovery After Photobleaching - methods
/ Humanities and Social Sciences
/ Humans
/ Lasers
/ Membrane Proteins - metabolism
/ Microscopy, Confocal - instrumentation
/ Microscopy, Confocal - methods
/ Microscopy, Fluorescence - instrumentation
/ Microscopy, Fluorescence - methods
/ Nuclear Envelope - metabolism
/ Proteins
/ Science
/ Single Molecule Imaging - instrumentation
