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Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP
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Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP
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Journal Article
Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP
2016
Overview
Little is known about how archaeal viruses perturb the transcription machinery of their hosts. Here we provide the first example of an archaeo-viral transcription factor that directly targets the host RNA polymerase (RNAP) and efficiently represses its activity. ORF145 from the temperate
Acidianus
two-tailed virus (ATV) forms a high-affinity complex with RNAP by binding inside the DNA-binding channel where it locks the flexible RNAP clamp in one position. This counteracts the formation of transcription pre-initiation complexes
in vitro
and represses abortive and productive transcription initiation, as well as elongation. Both host and viral promoters are subjected to ORF145 repression. Thus, ORF145 has the properties of a global transcription repressor and its overexpression is toxic for
Sulfolobus
. On the basis of its properties, we have re-named ORF145 RNAP Inhibitory Protein (RIP).
How archaeal viruses perturb host transcription machinery is poorly understood. Here, the authors provide evidence that the archaeo-viral transcription factor ORF145/RIP targets host RNA polymerase, repressing its activity.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/33
/ 38/1
/ 42/35
/ 82/16
/ 82/29
/ 82/80
/ 82/83
/ Biology
/ DNA-Directed RNA Polymerases - metabolism
/ Genes
/ Humanities and Social Sciences
/ Proteins
/ Science
/ Transcription Initiation, Genetic
/ Viruses
