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The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticola
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The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticola
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Journal Article
The major outer sheath protein forms distinct conformers and multimeric complexes in the outer membrane and periplasm of Treponema denticola
2017
Overview
The major outer sheath protein (MOSP) is a prominent constituent of the cell envelope of
Treponema denticola
(TDE) and one of its principal virulence determinants. Bioinformatics predicts that MOSP consists of N- and C-terminal domains, MOSP
N
and MOSP
C
. Biophysical analysis of constructs refolded
in vitro
demonstrated that MOSP
C
, previously shown to possess porin activity, forms amphiphilic trimers, while MOSP
N
forms an extended hydrophilic monomer. In TDE and
E. coli
expressing MOSP with a PelB signal sequence (PelB-MOSP), MOSP
C
is OM-embedded and surface-exposed, while MOSP
N
resides in the periplasm. Immunofluorescence assay, surface proteolysis, and novel cell fractionation schemes revealed that MOSP in TDE exists as outer membrane (OM) and periplasmic trimeric conformers; PelB-MOSP, in contrast, formed only OM-MOSP trimers. Although both conformers form hetero-oligomeric complexes in TDE, only OM-MOSP associates with dentilisin. Mass spectrometry (MS) indicated that OM-MOSP interacts with proteins in addition to dentilisin, most notably, oligopeptide-binding proteins (OBPs) and the β-barrel of BamA. MS also identified candidate partners for periplasmic MOSP, including TDE1658, a spirochete-specific SurA/PrsA ortholog. Collectively, our data suggest that MOSP destined for the TDE OM follows the canonical BAM pathway, while formation of a stable periplasmic conformer involves an export-related, folding pathway not present in
E. coli
.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/63
/ 82/1
/ 82/16
/ 82/29
/ 82/58
/ 82/80
/ 82/83
/ Bacterial Outer Membrane Proteins - metabolism
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - metabolism
/ E coli
/ Escherichia coli - metabolism
/ Escherichia coli Proteins - chemistry
/ Escherichia coli Proteins - metabolism
/ Humanities and Social Sciences
/ Science
