Journal Article

Gram-negative trimeric porins have specific LPS binding sites that are essential for porin biogenesis

Le Brun, Anton P., Baslé, Arnaud, van den Berg, Bert, Ridley, Helen, Arunmanee, Wanatchaporn, Solovyova, Alexandra S., Lakey, Jeremy H., Pathania, Monisha

2016

Overview

The outer membrane (OM) of gram-negative bacteria is an unusual asymmetric bilayer with an external monolayer of lipopolysaccharide (LPS) and an inner layer of phospholipids. The LPS layer is rigid and stabilized by divalent cation cross-links between phosphate groups on the core oligosaccharide regions. This means that the OM is robust and highly impermeable to toxins and antibiotics. During their biogenesis, OM proteins (OMPs), which function as transporters and receptors, must integrate into this ordered monolayer while preserving its impermeability. Here we reveal the specific interactions between the trimeric porins of Enterobacteriaceae and LPS. Isolated porins form complexes with variable numbers of LPS molecules, which are stabilized by calcium ions. In earlier studies, two high-affinity sites were predicted to contain groups of positively charged side chains. Mutation of these residues led to the loss of LPS binding and, in one site, also prevented trimerization of the porin, explaining the previously observed effect of LPS mutants on porin folding. The high-resolution X-ray crystal structure of a trimeric porin–LPS complex not only helps to explain the mutagenesis results but also reveals more complex, subtle porin–LPS interactions and a bridging calcium ion.

• Publisher: National Academy of Sciences
• Subject: Amino Acid Motifs / Amino Acid Substitution / Antibiotics / Bacteria / Binding Sites / Biological Sciences / Biosynthesis / Calcium / Calcium - chemistry / Calcium - metabolism / Cations, Divalent / Crystallography, X-Ray / Enterobacteriaceae / Escherichia coli - chemistry / Escherichia coli - genetics / Escherichia coli - metabolism / Gene Expression / Gram-negative bacteria / Lipopolysaccharides - chemistry / Lipopolysaccharides - metabolism / Membranes / Microbiology / Models, Molecular / Mutagenesis / Mutation / PNAS Plus / Porins - chemistry / Porins - genetics / Porins - metabolism / Protein Binding / Protein Conformation, alpha-Helical / Protein Conformation, beta-Strand / Protein Folding / Protein Interaction Domains and Motifs / Protein Multimerization / Proteins / Static Electricity / Toxins