Asset Details
Do you wish to reserve the book?
Using an intravenous catheter to carry out abdominal lavage in the gerbil
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Using an intravenous catheter to carry out abdominal lavage in the gerbil
Do you wish to request the book?
Using an intravenous catheter to carry out abdominal lavage in the gerbil
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Using an intravenous catheter to carry out abdominal lavage in the gerbil
2010
Overview
Abdominal lavage is used in laboratory rodents for a variety of applications but carries an inherent risk of abdominal organ laceration; therefore, personnel carrying out this procedure must have considerable expertise. In this paper, the authors describe an improved method for delivering sterile media to and collecting peritoneal fluids from dark-clawed Mongolian gerbils (
Meriones unguiculatus
) that had been peritoneally infected with filarial nematode parasites (genus
Brugia
). To carry out this gravity-assisted technique, the authors used a catheter to introduce sterile media into the peritoneal cavity of each gerbil and then to passively drain peritoneal fluid and larval worms for collection. Average fluid recovery was consistently greater when using this gravity-assisted method than when using aspiration. Larval parasites were recovered by both methods. To recover large volumes of fluid using the standard method of abdominal lavage, personnel typically must euthanize rodents. This gravity-assisted technique allows researchers to collect large numbers of parasite larvae without euthanizing gerbils.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Biomedical and Life Sciences
/ Brugia
/ Brugia - isolation & purification
/ Catheterization - veterinary
/ Complications and side effects
/ Diseases
/ Elephantiasis, Filarial - parasitology
/ Gerbils
/ gravity
/ Laboratory Animal Science - instrumentation
/ Laboratory Animal Science - methods
/ Male
/ methods
/ Parasitic Diseases, Animal - parasitology
