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Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
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Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
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Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity

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Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity
Journal Article

Formulation and characterization of Caesalpinia decapetala seed oil nanoemulsion: physicochemical properties, stability, and antibacterial activity

2025
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Overview
Nanoemulsions contain acceptable O/W or W/O dispersions, with droplet sizes ranging from 100 to 500 nm. In the current study, we formulated O/W nanoemulsion using Caesalpinia decapetala seed oil, Tween 20, and Tween 80 surfactant through ultrasonic and spontaneous emulsification. C. decapetala is a climbing shrub thorny tree. C. decapetala seed oil contains different chemical constituents. As a result, physicochemical properties of prepared nanoemulsion came to be 132.561 ± 0.491–290.033 ± 1.952 nm average particle size, 0.028 ± 0.038–0.301 ± 0.042 polydispersive index, - 32.274 to - 58 mV zeta potentials, 4.383–6.5 pH value, 3.922–5.247 mPa.s viscosity value with spherical shape, with excellent physical stability. Subsequently, gram-positive and gram-negative bacterial strains were employed to assess the nanoemulsion’s antibacterial efficacy. Therefore, the results indicate that C. decapetala seed oil nanoemulsion has excellent antibacterial activity on gram-positive and gram-negative bacteria strains. This nanoemulsion formulated using Tween 80 had higher inhibition zones like 13.5,13, 12, and 11 mm than the other methods on S. aureus , E.coli , S.pyogenes , and P. aeruginosa , respectively. Generally, smaller particle size, polydispersive index, stable surface charge, and low value of viscosity indicate that formulated nanoemulsion had better stability and bioavailability activity for antibacterial activity.