Journal Article

Photocatalytic antibacterial application of zinc oxide nanoparticles and self-assembled networks under dual UV irradiation for enhanced disinfection

Hwang, Woochul, Jin, Su-Eon, Hong, Seok Won, Jin, Jun Eon

2019

Overview

Zinc oxide (ZnO) nanoparticles and their networks have been developed for use in various applications such as gas sensors and semiconductors. In this study, their antibacterial activity against under dual ultraviolet (UV) irradiation for disinfection was investigated. ZnO nanoparticles were synthesized and immobilized onto silicon (Si) wafers by self-assembly. The physicochemical properties and antibacterial activity of ZnO nanoparticles and their networks were evaluated. Gene ontology was analyzed and toxicity levels were also monitored. Synthesized ZnO nanoparticles were spherical nanocrystals (<100 nm; Zn, 47%; O, 53%) that formed macro-mesoporous three-dimensional nanostructures on Si wafers in a concentration-dependent manner. ZnO nanoparticles and their networks on Si wafers had an excellent antibacterial activity against under dual UV irradiation (>3log CFU/mL). Specifically, arrayed ZnO nanoparticle networks showed superior activity compared with free synthesized ZnO nanoparticles. Oxidative stress-responsive proteins in were identified and categorized, which indicated antibacterial activity. Synthesized ZnO nanoparticles were less cytotoxic in HaCaT with an IC50 of 6.632 mg/mL, but phototoxic in Balb/c 3T3. The results suggested that ZnO nanoparticles and their networks can be promising photocatalytic antibiotics for use in next-generation disinfection systems. Their application could also be extended to industrial and clinical use as effective and safe photocatalytic antibiotics.

• Publisher: Taylor & Francis Ltd,Dove Press,Dove Medical Press
• Subject: Anti-Bacterial Agents - pharmacology / antibacterial / Antimicrobial agents / Catalysis / Cell Line / Crystallization / Cytotoxicity / disinfection / Disinfection - methods / Drug dosages / Drug resistance / dual UV / E coli / Escherichia coli - drug effects / Escherichia coli - radiation effects / Ethanol / Eye - cytology / Fourier transforms / Humans / immobilization / Metal oxides / Microbial Sensitivity Tests / Nanoparticles / Nanoparticles - chemistry / Nanoparticles - toxicity / Nanoparticles - ultrastructure / Original Research / Particle Size / Physicochemical properties / Porous materials / Scanning electron microscopy / Silicon - pharmacology / Skin / Skin - cytology / Software / Spectrum analysis / toxicity / Ultraviolet Rays / Zinc Oxide - pharmacology / Zinc Oxide - toxicity / zinc oxide nanoparticle / zinc oxide nanoparticles / Zinc oxides