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Double port injector device to reduce endothelial damage in DMEK
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Double port injector device to reduce endothelial damage in DMEK
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Journal Article
Double port injector device to reduce endothelial damage in DMEK
2014
Overview
Objective
To study endothelial injury from a newly designed asymmetric double port Descemet Membrane Endothelial Keratoplasty (DMEK) injector, both
ex-vivo
and in clinical practice.
Design
Laboratory investigation with an interventional case series study.
Method
Sixteen rabbit endothelial rolls were tested for injection using a no-touch technique. For each pair of rolls, one endothelial graft underwent injection with a single port Pasteur pipette twice, wheras the other was injected with a novel asymmetric double port injector with a larger diameter entry port than the exit port also twice. Each graft was stained with 4-6-diamidino-2-phenylinidole dihydrochloride and was counted under a fluorescence-inverted microscope before and after injection. The proportion of graft injury was calculated and the differences were analyzed. Subsequently, six patients requiring DMEK underwent surgery using this novel insertion device and endothelial cell loss was calculated 3 months after the surgery.
Results
After injection, the mean proportion of endothelial cell survival with the single port pipette was 78.8% (
n
=8; SD: ±20.9%), whereas the double port injector yielded a survival rate of 96.8% (
n
=8; SD: ±8.4%). This difference was statistically significant (
P
=0.008), representing less endothelial injury with the double port device. Early endothelial cell loss after 3 months in the DMEK patients was 26.1% (SD: ±6.1%).
Conclusion
In our injection model, using a double port injector created significantly less endothelial cell damage than with the single port pipette. Clinically, this device yielded early endothelial cell loss comparable to that of the series performed by experienced DMEK surgeons.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Aged
/ Animals
/ Corneal Endothelial Cell Loss - diagnosis
/ Descemet Stripping Endothelial Keratoplasty - instrumentation
/ Endothelium, Corneal - injuries
/ Endothelium, Corneal - pathology
/ Eye Injuries - prevention & control
/ Female
/ Humans
/ Injections - instrumentation
/ Male
/ Medicine
/ Pharmaceutical Sciences/Technology
/ Rabbits
/ Surgery
