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Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
by
Medda, Rebecca
, Schönle, Andreas
, Geisler, Claudia
, Wenzel, Dirk
, Stiel, Andre C.
, Hell, Stefan W.
, Bock, Hannes
, Egner, Alexander
, von Middendorff, Claas
, Jakobs, Stefan
, Andresen, Martin
, Eggeling, Christian
in
Animals
/ Biophysics
/ Cell Line
/ Cellular biology
/ Escherichia coli - cytology
/ Fluorescence
/ Fluorescence microscopy
/ Fluorescent Dyes - metabolism
/ Localization
/ Macropodidae
/ Mammals
/ Microscopy
/ Microscopy, Fluorescence - instrumentation
/ Nanocomposites
/ Nanomaterials
/ Nanostructure
/ Nanotechnology
/ Position (location)
/ Recording
/ Spectroscopy, Imaging, Other Techniques
2007
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Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
by
Medda, Rebecca
, Schönle, Andreas
, Geisler, Claudia
, Wenzel, Dirk
, Stiel, Andre C.
, Hell, Stefan W.
, Bock, Hannes
, Egner, Alexander
, von Middendorff, Claas
, Jakobs, Stefan
, Andresen, Martin
, Eggeling, Christian
in
Animals
/ Biophysics
/ Cell Line
/ Cellular biology
/ Escherichia coli - cytology
/ Fluorescence
/ Fluorescence microscopy
/ Fluorescent Dyes - metabolism
/ Localization
/ Macropodidae
/ Mammals
/ Microscopy
/ Microscopy, Fluorescence - instrumentation
/ Nanocomposites
/ Nanomaterials
/ Nanostructure
/ Nanotechnology
/ Position (location)
/ Recording
/ Spectroscopy, Imaging, Other Techniques
2007
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Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
by
Medda, Rebecca
, Schönle, Andreas
, Geisler, Claudia
, Wenzel, Dirk
, Stiel, Andre C.
, Hell, Stefan W.
, Bock, Hannes
, Egner, Alexander
, von Middendorff, Claas
, Jakobs, Stefan
, Andresen, Martin
, Eggeling, Christian
in
Animals
/ Biophysics
/ Cell Line
/ Cellular biology
/ Escherichia coli - cytology
/ Fluorescence
/ Fluorescence microscopy
/ Fluorescent Dyes - metabolism
/ Localization
/ Macropodidae
/ Mammals
/ Microscopy
/ Microscopy, Fluorescence - instrumentation
/ Nanocomposites
/ Nanomaterials
/ Nanostructure
/ Nanotechnology
/ Position (location)
/ Recording
/ Spectroscopy, Imaging, Other Techniques
2007
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Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
Journal Article
Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters
2007
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Overview
We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40
nm.
Publisher
Elsevier Inc,Biophysical Society,The Biophysical Society
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