Journal Article

Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose

Oboh, Ganiyu, Ogunbadejo, Mariam Damilola, Ogunsuyi, Opeyemi Babatunde, Adefegha, Stephen Adeniyi

2016

Overview

Acarbose is an antidiabetic drug which acts by inhibiting α-amylase and α-glucosidase activities but with deleterious side effects. Gallic acid (GA) is a phenolic acid that is widespread in plant foods. We therefore investigated the influence of GA on α-amylase and α-glucosidase inhibitory properties of acarbose (in vitro). Aqueous solutions of acarbose and GA were prepared to a final concentration of 25μM each. Thereafter, mixtures of the samples (50% acarbose + 50% GA; 75% acarbose+25% GA; and 25% acarbose+75% GA) were prepared. The results revealed that the combination of 50% acarbose and 50% GA showed the highest α-glucosidase inhibitory effect, while 75% acarbose+25% GA showed the highest α-amylase inhibitory effect. Furthermore, all the samples caused the inhibition of Fe2+-induced lipid peroxidation (in vitro) in rat pancreatic tissue homogenate, with the combination of 50% acarbose and 50% GA causing the highest inhibition. All the samples also showed antioxidant properties (reducing property, 2,2'-azino-bis (-3-ethylbenzthiazoline-6-sulphonate [ABTS*] and 1,1-diphenyl-2-picrylhydrazyl [DPPH] free radicals scavenging abilities, and Fe2+ chelating ability). Therefore, combinations of GA with acarbose could be employed as antidiabetic therapy, with a possible reduction of side effects of acarbose; nevertheless, the combination of 50% acarbose and 50% GA seems the best. [Display omitted]

• Publisher: Elsevier B.V,Food and Drug Administration,Taiwan Food and Drug Administration
• Subject: 2,2-diphenyl-1-picrylhydrazyl / Acarbose / Acarbose - pharmacology / adverse effects / alpha-amylase / alpha-Amylases / alpha-glucosidase / alpha-Glucosidases / Amylases / antidiabetic / Antidiabetics / antioxidant activity / Antioxidants / aqueous solutions / Chelation / Chemicals / combination therapy / Diabetes / Diabetes mellitus / Enzyme Inhibitors - pharmacology / Enzymes / Food plants / Free radicals / Gallic Acid / Glucose / Glucosidase / Glycoside Hydrolase Inhibitors / Hyperglycemia / hypoglycemic agents / Inhibitory Concentration 50 / Insulin resistance / Iron / Lipid peroxidation / Original / Oxidative stress / Pancreas / Peroxidation / Phenolic acids / Phenols / Plant Extracts / plant foods / Potassium / Properties (attributes) / rats / Side effects / therapeutics