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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
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Journal Article
CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
2017
Overview
Editorial Summary
Most microbial biosynthetic gene clusters are inactive under laboratory culture conditions. A CRISPR–Cas9 genome-editing approach in
Streptomyces
species enables the targeted activation of silent gene clusters and production of encoded natural products.
Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five
Streptomyces
species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in
Streptomyces viridochromogenes
. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.
