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Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
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Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
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Journal Article
Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
2010
Overview
High-density cell culture is pivotal for the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). Two high-density cell culture systems, micromass and pellet culture, have been used to induce chondrogenic differentiation of hMSCs. In micromass culture, the induced-cartilage tissues were larger, more homogenous and enriched in cartilage-specific collagen II but the fibrocartilage-like feature, collagen I, and hypertrophic chondrocyte feature, collagen X, were markedly decreased compared to those in pellet culture. Furthermore, real time RT-PCR analysis demonstrated that collagen II and aggrecan mRNA were up-regulated while collagen X and collagen I mRNA were down-regulated in micromass culture. Thus, the micromass culture system is a promising tool for in vitro chondrogenic studies.
Publisher
Dordrecht : Springer Netherlands,Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ collagen
/ Collagen Type I - biosynthesis
/ Collagen Type II - biosynthesis
/ Collagen Type X - biosynthesis
/ Culture
/ Fundamental and applied biological sciences. Psychology
/ Human
/ Humans
/ Mesenchymal Stromal Cells - physiology
/ Pellets
/ quantitative polymerase chain reaction
/ reverse transcriptase polymerase chain reaction
/ RNA, Messenger - biosynthesis
/ tissues
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