Journal Article

Zingiber officinale Roscoe Rhizome Extract Exerts Senomorphic and Anti-Inflammatory Activities on Human Endothelial Cells

Galeotti, Nicoletta, Borgonetti, Vittoria, Olivieri, Fabiola, Silvestrini, Andrea, Matacchione, Giulia, Ojetti, Marta, Ramini, Deborah

2023

Overview

Aging is related to a low-grade and sterile inflammation called inflammaging, recognized as the main risk factor for age-related disease (ARD) development. Inflammaging is fostered by the repeated activation of immune cells, as well as by the accumulation of senescent cells. Recently, a number of natural compounds have gained attention to be tested as anti-aging therapies, based on their anti-inflammatory activity and/or ability to reduce the pro-inflammatory secretome of senescent cells (senomorphyc activity). Here, we investigated the anti-inflammatory and senomorphic properties of an Asian-native Zingiber officinale Roscoe extract (ZOE), commonly consumed as a food spice and herbal medicine. We employed two models of primary endothelial cells (HUVECs), such as the replicative-senescence and LPS-induced response, to investigate the anti-inflammatory/senomorphic effect of ZOE, and one cellular model of neuroinflammation, i.e., immortalized murine microglial cells (BV2). First, we found that the ZOE treatment induced the inhibition of NF-kB activation in BV2 cells. Among the constituents of ZOE, we showed that the terpenoid-enriched fraction (ZTE) was the component able to counteract the phosphorylation of NF-kB(p65), while 6-gingerol (GIN) and 6-shogaol (SHO) did not produce any significant effect. Further, we observed that the treatment with 10 µg/mL of ZOE exerted anti-inflammatory activity on LPS-stimulated young (y)HUVEC and senomorphyc activity on replicative senescent (s)HUVEC, significantly reducing the expression levels of IL-1β, TNF -α, IL-8, MCP-1, and ICAM-1. Moreover, the ZTE treatment was able to significantly reduce the IL-8 levels secreted in the medium of both LPS-stimulated yHUVEC and sHUVEC. Overall, our data suggest a potential protective role of ZOE on neuroinflammation and endothelial inflammation/activation, thus suggesting its potential relevance in delaying/postponing ARD development and progression, characterized by endothelial dysfunction.

• Publisher: MDPI AG,MDPI
• Subject: Aging / anti-inflammatory activity / Anti-inflammatory agents / Cell activation / Cell culture / Cell cycle / Cytotoxicity / Dementia disorders / Diabetes mellitus / Endothelial cells / Genotype & phenotype / Herbal medicine / herbal medicines / humans / inflammaging / Inflammation / Intercellular adhesion molecule 1 / Interleukin 8 / Lipopolysaccharides / mice / Microglial cells / MicroRNAs / Monocyte chemoattractant protein 1 / natural compounds / neuroinflammation / NF-κB protein / Phosphorylation / protective effect / Rhizomes / Risk factors / Secretome / Senescence / senomorphics / spices / transcription factor NF-kappa B / Zingiber officinale / Zingiber officinalis roscoe