Journal Article

Extracellular HIV-1 Tat induces human beta-defensin-2 production via NF-kappaB/AP-1 dependent pathways in human B cells

Kwon, D.J., Hallym University, Chuncheon, Republic of Korea, Ju, S.M., Hallym University, Chuncheon, Republic of Korea, Choi, S.Y., Hallym University, Chuncheon, Republic of Korea, Park, J.S., Hallym University, Chuncheon, Republic of Korea, Goh, A.R., Hallym University, Chuncheon, Republic of Korea, Youn, G.S., Hallym University, Chuncheon, Republic of Korea, Kwon, H.J., Hallym University, Chuncheon, Republic of Korea, Bae, Y.S., Sungkyunkwan University, Suwon, Republic of Korea

2012

Overview

Defensins, a family of antimicrobial peptides, are one of the first lines of host defense. Human beta-defensins (hBD) such as hBD-2 and -3 have anti-HIV activity. Previous studies have shown that HIV-1 virion can induce the expression of hBD, although the exact components of HIV-1 virion that are responsible for hBD expression have not yet been elucidated. In this study, we examined the effect of HIV-1 Tat on the expression of hBD in B cells. Stimulation of B cells with HIV-1 Tat protein significantly increased the mRNA and protein levels of hBD-2. HIV-1 Tat also induced the activation of a reporter gene for hBD-2 in a dose-dependent manner in B cells. Pretreatment of B cells with a JNK inhibitor suppressed HIV-1 Tat-induced hBD-2 expression. Pretreatment of B cells with AP-1 inhibitors or NF-κB inhibitors led to a decrease in HIV-1 Tat-induced protein and mRNA expression of hBD-2. Taken together, our results indicate that HIV-1 Tat can up-regulate the expression of hBD-2 via JNK-NF-κB/AP-1-dependent pathways in human B cells.

• Publisher: Korean Society for Molecular and Cellular Biology,Korea Society for Molecular and Cellular Biology,한국분자세포생물학회
• Subject: AP-1 / beta-defensins / Biochemistry / Biomedical and Life Sciences / Biomedicine / Biotechnology / Cell Biology / HUMAN IMMUNODEFICIENCY VIRUS / Life Sciences / LINFOCITOS / LYMPHOCYTE / LYMPHOCYTES / MAPK / NF-kappa B / Tat / VIRUS DE L'IMMUNODEFICIENCE HUMAINE / VIRUS DE LA INMUNODEFICIENCIA HUMANA / 생물학