Journal Article

Is the Poly (L- Lactide- Co– Caprolactone) Nanofibrous Membrane Suitable for Urinary Bladder Regeneration?

Bodnar, Magdalena, Warda, Karolina, Kowalczyk, Tomasz, Pokrywczynska, Marta, Debski, Robert, Frontczak-Baniewicz, Malgorzata, Chmielewski, Tomasz, Nakielski, Pawel, Jundzill, Arkadiusz, Krzyzanowska, Sandra, Mikułowski, Grzegorz, Kowalewski, Tomasz A., Marszalek, Andrzej, Buchholz, Lukasz, Drewa, Tomasz, Nowacki, Maciej, Adamowicz, Jan, Rasmus, Marta

2014

Overview

The purpose of this study was to compare: a new five-layered poly (L-lactide-co-caprolactone) (PLC) membrane and small intestinal submucosa (SIS) as a control in rat urinary bladder wall regeneration. The five-layered poly (L-lactide-co-caprolactone) membrane was prepared by an electrospinning process. Adipose tissue was harvested from five 8-week old male Wistar rats. Adipose derived stem cells (ADSCs) were seeded in a density of 3×10(6) cells/cm2 onto PLC membrane and SIS scaffolds, and cultured for 5-7 days in the stem cell culture medium. Twenty male Wistar rats were randomly divided into five equal groups. Augmentation cystoplasty was performed in a previously created dome defect. Groups: (I) PLC+ 3×10(6)ADSCs; (II) SIS+ 3×10(6)ADSCs; (III) PLC; (IV) SIS; (V) control. Cystography was performed after three months. The reconstructed urinary bladders were evaluated in H&E and Masson's trichrome staining. Regeneration of all components of the normal urinary bladder wall was observed in bladders augmented with cell-seeded SIS matrices. The urinary bladders augmented with SIS matrices without cells showed fibrosis and graft contraction. Bladder augmentation with the PLC membrane led to numerous undesirable events including: bladder wall perforation, fistula or diverticula formation, and incorporation of the reconstructed wall into the bladder lumen. The new five-layered poly (L-lactide-co-caprolactone) membrane possesses poorer potential for regenerating the urinary bladder wall compared with SIS scaffold.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acids / Adipocytes - cytology / Adipocytes - physiology / Adipose tissue / Animals / Augmentation / Biology and Life Sciences / Biomechanics / Biomedical materials / Bladder / Cell culture / Cell Differentiation / Cell division / Cell Proliferation / Cells, Cultured / Contraction / Extracellular matrix / Fibrosis / Fistulae / Intestinal Mucosa - cytology / Intestinal Mucosa - physiology / Intestine / Kopernik, Mikolaj (Nicolaus Copernicus) (1473-1543) / Laboratory animals / Male / Medicine / Medicine and Health Sciences / Membranes / Membranes, Artificial / Perforation / Physics / Polyesters - chemistry / Programmable logic controllers / Rats / Rats, Wistar / Regeneration / Scaffolds / Stem cell transplantation / Stem cells / Tissue engineering / Tissue Engineering - methods / Tissue Scaffolds / Urinary bladder / Urinary Bladder - physiology / Urinary Bladder - surgery / Urine / Urology