Journal Article

Direct comparison of the four aldehyde oxidase enzymes present in mouse gives insight into their substrate specificities

Leimkühler, Silke, Kücükgöze, Gökhan

2018

Overview

Mammalian aldehyde oxidases (AOXs) are molybdo-flavoenzymes which are present in many tissues in various mammalian species, including humans and rodents. Different species contain a different number of AOX isoforms. In particular, the reasons why mammals other than humans express a multiplicity of tissue-specific AOX enzymes is unknown. In mouse, the isoforms mAOX1, mAOX3, mAOX4 and mAOX2 are present. We previously established a codon-optimized heterologous expression systems for the mAOX1-4 isoforms in Escherichia coli that gives yield to sufficient amounts of active protein for kinetic characterizations and sets the basis in this study for site-directed mutagenesis and structure-function studies. A direct and simultaneous comparison of the enzymatic properties and characteristics of the four enzymes on a larger number of substrates has never been performed. Here, thirty different structurally related aromatic, aliphatic and N-heterocyclic compounds were used as substrates, and the kinetic parameters of all four mAOX enzymes were directly compared. The results show that especially mAOX4 displays a higher substrate selectivity, while no major differences between mAOX1, mAOX2 and mAOX3 were identified. Generally, mAOX1 was the enzyme with the highest catalytic turnover for most substrates. To understand the factors that contribute to the substrate specificity of mAOX4, site-directed mutagenesis was applied to substitute amino acids in the substrate-binding funnel by the ones present in mAOX1, mAOX3, and mAOX2. An increase in activity was obtained by the amino acid exchange M1088V in the active site identified to be specific for mAOX4, to the amino acid identified in mAOX3.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Aldehyde oxidase / Aldehyde Oxidase - chemistry / Aldehyde Oxidase - genetics / Aldehyde Oxidase - metabolism / Aldehyde Oxidoreductases - chemistry / Aldehyde Oxidoreductases - genetics / Aldehyde Oxidoreductases - metabolism / Aldehydes / Aldehydes - chemistry / Aldehydes - metabolism / Aliphatic compounds / Amino Acid Substitution / Amino acids / Animals / Aromatic compounds / Benzaldehydes - chemistry / Benzaldehydes - metabolism / Binding sites / Biochemistry / Biology and Life Sciences / Catalytic Domain - genetics / Crystal structure / E coli / Enzymes / Flavoproteins - chemistry / Flavoproteins - genetics / Flavoproteins - metabolism / Heterocyclic compounds / Heterocyclic Compounds - chemistry / Heterocyclic Compounds - metabolism / Isoenzymes - chemistry / Isoenzymes - genetics / Isoenzymes - metabolism / Isoforms / Kinetics / Mammals / Metabolism / Mice / Models, Molecular / Molybdenum / Mutagenesis, Site-Directed / Physical Sciences / Proteins / Recombinant Proteins - chemistry / Recombinant Proteins - genetics / Recombinant Proteins - metabolism / Research and Analysis Methods / Selectivity / Site-directed mutagenesis / Structure-function relationships / Studies / Substrate Specificity / Substrates