Journal Article

Serum IgG Mediates Mucosal Immunity against Rotavirus Infection

Jiang, Baoming, Almond, Jeffrey W., Robbins, John B., McClure, Harold M., Glass, Roger I., Westerman, Larry E.

2005

Overview

We evaluated the protective role of passively transferred circulating antibodies in protecting non-human primates against experimental rotavirus infection. Pooled sera with rotavirus-specific IgG titers that were either high (1:10,000), intermediate (1:300), or negative (<1:25) were infused i.v. into naïve pigtailed macaques (ages 3-6 months). Rotavirus-specific IgG could be detected in the sera at 18 h in all animals infused with antibody-containing serum, and fecal IgG titers could be detected only in animals given high-titer pooled sera. When orally challenged with 106fluorescent-forming units of a simian rotavirus strain, YK-1, at 18 h after serum transfer, control animals shed virus starting 1-3 days after challenge and continued to shed virus at high titers for 6-8 days, whereas passively immunized macaques did not shed virus or had delayed shedding at low titers for only a limited time. The observation that passively transferred antibodies can suppress or delay viral infection in rotavirus-challenged pigtailed macaques has important implications for the design and testing of parenteral candidate rotavirus vaccines.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Animals / Antibodies / Antibodies, Viral - immunology / Antigens / Biological Sciences / Disease models / Feces - chemistry / Immune system / Immunity / Immunity, Mucosal - immunology / Immunization / Immunoglobulin G - blood / Immunoglobulin G - immunology / Infections / Macaca nemestrina / Neutralization Tests / Passive immunization / Primates / Rotavirus / Rotavirus - immunology / Rotavirus infections / Rotavirus Infections - immunology / Vaccination / Virus Shedding - immunology / Viruses