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Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood
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Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood
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Journal Article
Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood
2013
Overview
The enzyme-linked immunospot (ELISPOT) assay was originally developed to enumerate antigen-specific antibody-secreting cells (ASCs), and has subsequently been adapted for various applications, including the detection cytokine-secreting cells. Owing to its exceptionally high sensitivity, the ELISPOT has proven to be especially useful for detecting discrete populations of active cells (e.g., antigen-specific cells). Because of its versatility, the ELISPOT assay is used for a wide range of applications, including clonal analyses of immune responses after vaccination or after immunotherapy. Here we describe standard protocols for the detection of human ASCs specific to virtually any vaccine antigen after enrichment of circulating plasmablasts. In addition, a protocol is described for the measurement of mucosal ASC responses after prior immunomagnetic enrichment of mucosally derived blood lymphocytes. The protocols described allow rapid (∼6–8 h) detection of specific ASCs in small (1–2 ml) samples of blood and can be performed in resource-poor settings.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 50
/ 67/18
/ 96/12
/ Antibody-Producing Cells - immunology
/ Antigens
/ Blood
/ Computational Biology/Bioinformatics
/ Enzyme-linked immunosorbent assay
/ Enzyme-Linked Immunospot Assay - methods
/ Enzymes
/ Humans
/ Immunity, Humoral - immunology
/ Methods
/ Plasma
/ Protocol
/ Serology
/ Vaccines
